Figure 8: Northern blot analysis of human NF-1 neurogenic sarcoma cell line (ST88-14) and human astrocytoma cell lines for VEGF mRNA expression. The NF-1 neurogenic sarcoma cell line, whose Ras activity has been documented to be elevated by other labs, expresses comparable levels of VEGF mRNA to the astrocytoma cells, whose increased Ras activity and inter-relationship to VEGF secretion is documented (see Figures 5, 11, 12). Effect of inhibiting Ras activity on cellular proliferation and VEGF secretion by the NF-1 neurogenic sarcoma cells are ongoing.